1.1 To lay down a procedure for Operation of HPLC (Shimadzu with millennium software).Ģ.1 This SOP is applicable for Operation of HPLC (Shimadzu with millennium software) in Quality control department.ĥ.1 Connect the instrument to 230 volts A/C power supply.ĥ.3 Connect the required column as per standard test procedure for the sample analysis and column shall be equilibrated by using either water: methanol (50:50) or water: Acetonitrile or ratio.ĥ.4 Prepare the prescribed mobile phase as per testing procedure and fill the reservoirs.ĥ.5 Open the drain valve of the pumps and press purge button to flush the lines with mobile phase.ĥ.6 After purging close the drain valve of the pump.ĥ.7 Set the pressure control to the maximum by using func key.ĥ.8 Set the concentration of pump-A &B in the conc mode of pump-A.ĥ.9 If the testing program is gradient enter the time events and conc.